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This experimental study investigates the impact of conversational AI (ConvAI) on students’ academic motivation and conceptual change in algebra learning. A between-subjects design with 151 participants assessed the effectiveness of ConvAI in enhancing mastery approach, self-efficacy, and conceptual understanding compared to traditional methods. Results from two-way MANCOVA and ANCOVA analyses reveal significant increases in academic motivation within the ConvAI group, particularly in areas of mastery approach and self-efficacy. However, conceptual change did not significantly differ overall between groups, though it was moderated by individual differences in academic motivation. Our open-sourced ConvAI implementations and study findings provided support and implications for educational practitioners and researchers to design and develop pedagogically meaningful ConvAI in math learning.