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The integration of Artificial Intelligence (AI) in education has increased teaching anxiety among pre-service teachers, driven by concerns about job security, ethical implications, and technology integration challenges. This study investigates how digital self-efficacy and social-emotional competence influence teaching anxiety in the age of AI, with demographic factors such as gender, teaching experience, AI familiarity, and educational discipline as potential moderators. A quantitative survey of 600 pre-service teachers in China will utilize validated and translated scales, confirmed for reliability in a pilot study (Cronbach’s alpha: 0.81–0.87). Findings are expected to provide a valuable understanding of how to reduce teaching anxiety through enhanced digital self-efficacy and social-emotional learning, providing practical implications for teacher education programs to better prepare educators for AI-integrated classrooms.