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In the context of inclusive education, differentiated instruction ensures students with special needs can achieve comprehensive development. The study of 650 Chinese mainstream teachers found a positive link between inclusive education climate and differentiated instruction (β=0.16, SE=0.05, 95% BootstrapCIs [0.07, 0.26]), mediated by a chain of factors including efficacy for inclusive practices (β=0.24, SE=0.04, 95% BootstrapCIs [0.15, 0.33]), attitudes towards inclusive education (β=0.34, SE=0.07, 95% BootstrapCIs [0.20, 0.49]), and their chain mediating effect (β=0.07, SE=0.02, 95% BootstrapCIs [0.04, 0.12]). The study suggested creating a positive climate, providing resources, and enhancing teachers' efficacy and attitudes to facilitate differentiated instruction.