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Through the lens of Social Cognitive Career Theory, this paper examines the career outcome and skill preparedness of biology PhD graduates. Findings reveal that graduates reported strong self-efficacy in research skills but identified significant gaps in business and advanced data competencies, while many viewed postdoctoral training as a necessary step regardless of long-term career goals. Additionally, while Principal Investigators were generally supportive, they often lacked the resources or networks to guide non-academic transitions. Career decisions were also shaped by outcome expectations, with both extrinsic factors and intrinsic motivations influencing career choices. Our findings offer evidence-based implications to support doctoral education that better prepares life sciences PhDs for a range of careers.