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Pre-service teacher communication training faces challenges in scalability and individualization. This study integrates Carkhuff’s interpersonal communication theory with self-regulated learning (SRL) theory to construct a three-phase training framework. A multi-agent system coordinated role-play, task management, and feedback to support complex skills development. In a nine-week quasi-experimental study, 35 pre-service teachers participated, with eye-tracking capturing learning processes. Results showed sustained development in the low self-efficacy group and cognitive recalibration in the high self-efficacy group. Delayed effect emerged in supportive communication and perspective taking, with eye-tracking revealing distinct attention strategies. Findings support individualized teacher education and interdisciplinary integration of educational technology and learning sciences.