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This study investigates how undergraduate biology students engage with generative AI (ChatGPT) through self-directed questioning. Using the ICAP framework, we analyzed 784 student-chatbot interactions to examine question types, transition patterns, and differences across performance levels. Results show that longer conversations foster more Constructive and Interactive questioning, especially among higher-performing students. Passive questioning dominated shorter exchanges and lower-performing students. While ChatGPT responses were accurate and specific, their instructional helpfulness varied. These findings highlight generative questioning as a key marker of meaningful learning with large language models.