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Assessing instructional quality provides valuable insights into teaching and learning. While video-based classroom observation is a well-established method for analyzing teacher–student interactions, it requires physical presence and adherence to data protection rules. Virtual reality (VR) offers a promising alternative by simulating realistic classroom scenarios. However, it remains unclear whether instructional quality can be validly assessed in such environments using standardized observation tools. In this study, 59 preservice teachers participated in VR-based teaching simulations, which were video recorded and analyzed using the Classroom Assessment Scoring System–Secondary (CLASS-S). A confirmatory factor analysis tested the validity of CLASS-S in this context. Results showed acceptable model fit, supporting its use for evaluating instructional quality in VR settings and enhancing teacher education.