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Motivation plays a key role in persistence in science fields. This study examined the development of science interest and self-efficacy among undergraduates using Growth Mixture Modeling to identify distinct motivational trajectories. Three developmental patterns emerged, revealing meaningful heterogeneity. Notably, one group began college with moderately high motivation but experienced a consistent decline. First-generation students were significantly more likely to follow this pattern, suggesting the need for more sustained support through college. In contrast, students with the highest initial interest and self-efficacy were more likely to pursue science-related careers and remain engaged in science activities years after graduation. Altogether, these findings highlight the importance of exploring heterogeneous patterns in developmental trajectories to identify and support students who need it most.