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To address the need for scalable and valid assessments in integrated STEM (iSTEM) education, this study examines whether students' perceptions of iSTEM instruction can be reliably and validly captured using single-item formats. Based on a multidimensional iSTEM framework, it assesses test–retest reliability, convergent validity, and construct validity in a large-scale, longitudinal sample of over 3,000 Austrian middle school students from a national iSTEM pilot. Findings show moderate to strong correlations with multi-item scales and meaningful links to motivational-affective outcomes, though test–retest reliability remains limited. The results highlight both the potential and current limitations of single-item instruments and emphasize the need to balance psychometric quality with practical feasibility in large-scale STEM research.