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The purpose of this study was to explore Intelligent Tutoring Systems (ITS), specifically the YinKe and EarMaster applications, in enhancing students’ sight-singing and aural skills. A quasi-experimental design was adopted, involving 130 undergraduates who were assigned to traditional teaching, YinKe, and EarMaster groups. The results from MANCOVA revealed that both experimental groups significantly outperformed the control group in sight-singing skill, aural skill, and rhythmic dictation, with the YinKe showing greater effectiveness in aural skill and rhythmic dictation compared to EarMaster. In interval identification and harmonic recognition, the YinKe group performed better than the traditional teaching group, while no significant differences were observed between the EarMaster group and either the YinKe group or the traditional teaching group.