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Rural teachers often face professional isolation, which limits access to effective professional development, especially in three-dimensional science instruction. This study examines how Technology-Mediated Lesson Study (TMLS), a collaborative, video-based professional learning model, helps four rural biology teachers improve their ability to teach using three-dimensional science principles. Using an ecological framework, the research demonstrates how TMLS fosters teacher growth and capacity across personal, social, and contextual domains. Over the course of a year, teachers increased their content knowledge, adopted student-centered strategies, and grew in their pedagogical understanding. The findings suggest that TMLS effectively reduces rural isolation and supports ongoing professional development in three-dimensional science, providing a practical approach to professional development for isolated educators across various disciplines.