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This study investigates preservice teachers’ knowledge and use of Generative AI in lesson plan development through the TPACK framework. The study also aims to develop effective instructional interventions through formative research to support their use of AI in teaching practices. A total of 137 preservice teachers participated in AI-integrated course activities, generating 203 prompts in chatlogs. The results showed limited interaction and weak TPACK-related skills in the first phase. After introducing instructional guidance in the second phase, 66 preservice teachers demonstrated more meaningful engagement and improved integration of technology, content, and pedagogy. The study highlights the importance of structured scaffolding when guiding preservice teachers to engage in more effective AI use to enhance their TPACK in lesson plan development.