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This study explores how middle school science teachers incorporate disciplinary literacy strategies into inquiry-based instruction through a three-year professional development (PD) program. This study draws on an integrative disciplinary literacy framework including writing-to-learn, reading-to-learn, discussion, and multimodality. We analyzed 18 lesson videos taught by three middle school science teachers across three PD phases. Results showed that teachers shifted from low-level literacy activities, predominant teacher-led discourse, to more reflective, collaborative, and multimodal tasks, and student-led discourse. Teachers also integrated more higher-order literacy and peer discourse in guided inquiry over time, where students engaged in evidence-based arguments, data analysis, and dialogic discussions. Our findings demonstrate the effectiveness of three-year teacher PD in developing contextualized science lessons with integration of disciplinary literacy.