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Background: Retaining teachers beyond their novice years in order for them to become more experienced is believed to be a key component of successful student learning and school effectiveness. Purpose: This study aimed to develop and validate an ecological school culture index (ESCI) for predicting novice teachers’ retention. Method: A mix-method approach was used. 52 novice teachers, teachers and principals were interviewed and 1570 teachers filled out validated questionnaires. Findings: Findings from analyses performed at the individual and school levels using MPLUS, indicated a three-factor model of ESCI comprising the following factors: Collegial relationships between teachers, Mentoring policies, and Principal-teacher relationships. ESCI showed factorial validity as well as incremental predictive validity. Importantly, ESC was found to predict novice teacher retention.