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In western China, some preschoolers start boarding as young as 3-year-old and stay at preschools for 4 or 5 consecutive days weekly. The current study examined the impact of boarding on preschoolers’ behavioral adjustments and stress responses reflected by cortisol and saliva alpha-amylase (sAA) during the transition to preschool, both in terms of the average stress and the changing trajectory over time. Saliva samples of 61 preschoolers (boarders = 31, non-boarders = 30) were collected over a 12-week period upon entry of preschools. Primary caregivers reported demographics, behavior problems, and school refusal. Boarders experience a larger morning-to-afternoon cortisol rise than non-boarders. Boarding moderated the change of daily sAA level over time. Boarder also exhibited more school refusal behaviors.