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We conducted a pretest-posttest design experiment across multiple sections of a calculus course in a large public university in the United States to address this challenge. Our goal was to test the effects of the game Variant: Limits (Author et al., 2017) on the requisite calculus content learning outcomes of STEM majors. After propensity score matching, 111 reasonably well-matched pairs of game completers and control participants (n=222) were identified as the present study's analytic sample. We calculated the post treatment mean difference effect size (d=0.73, 95% CI=[0.46, 1.01]). The mean difference effect size indicates that the Variant impact: Limits game had a large and statistically significant effect on test scores. Implications for increased game-based instruction are provided.