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How does organizational composition affect the practice of cyberspace operations? To what extent do organizational subcultures matter to the study of cyberspace? In this paper, I argue that the composition of military organizations will influence how those organizations pursue and integrate cyberspace operations into military campaigns. Specifically, I argue that operational backgrounds – by which I mean the training, accumulated skills, organizational expertise, and related cultural instincts derived from one’s past professional experiences and codified in organizational subcultures – can, under certain conditions, function as the primary determinant of how organizations interpret new problems. These interpretations will affect the trajectory of the operations that follow. I test this argument by comparing the development of cyberspace doctrine in the U.S. and Russian Armies. I find that the presence or absence of certain organizational subcultures had a decisive impact on the how these organizations conceived of cyberspace operations and subsequently pursued cyberspace capability. As a result, I argue that organizational composition is of decisive importance to the study of cyberspace and to understanding the outcomes of cyberspace operations.