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The effective management of HIV is time intensive and constrains daily activities, including employment, household chores, and leisure. Yet our knowledge about general time use and its consequences among people with HIV is limited. Using a population-representative sample of young women in Malawi who are most affected by the pandemic, we explore 24-hour time use among HIV+ and HIV- women. We found that there is no absolute mean time difference in all activities except for sleeping and healthcare activities. However, the concentration of activities throughout the day differs by HIV status. We further found that the determinants of time use differ by HIV status. Wealth and age predict HIV- women’s participation in economic and social activities, but not for HIV+ women. We suggest that studying time use differences by HIV status allows us to better understand the social consequences of living with HIV in resource-poor settings.