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While Sexual Assault Kit Initiative (SAKI) projects have expanded nationwide, a paucity of studies has examined the predictors of sexual assault kit (SAK) testing. The current study presents a comparative analysis of cases of sexual assault reported to Anoka County, MN Sherrif’s Office associated with SAKs that were tested during the investigation and those that were not tested. The sample includes 173 cases of sexual assault with a tested SAK (i.e., treatment group) and 406 cases with a (previously) untested SAK (i.e., control group). Data stemmed from sexual assault investigative case files, forensic reports, and summary notes from the victim advocate. Bivariate and multivariate analyses examined differences between the treatment and control groups in case, victim, and suspect characteristics. Findings show significant differences between cases with a tested versus untested SAK that align with legal characteristics (e.g., time to report, victim injury) and perceived evidentiary value (e.g., victim offender relationship) rather than victim and suspect characteristics and behaviors. Empirical examinations regarding predictors of SAK testing is critical for a comprehensive review of sexual assault case processing in a jurisdiction and to inform changes in policy and practice.