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The purpose of this study is to examine the cultural difference in the portrayal of main antagonists between Walt Disney and Studio Ghibli animated films. We analyze whether main antagonists engage in both prosocial and aggressive behaviors (including physical, indirect, and verbal aggression). Moreover, we explore demographics of main antagonists and context of each aggressive and prosocial act initiated by a main antagonist. Results from a content analysis showed that main antagonists in Studio Ghibli tend to participate in both prosocial and aggressive behaviors, while those in Walt Disney tend to commit only aggressive behaviors to harm others. This study concludes that the difference in the portrayal of main antagonists between Walt Disney and Studio Ghibli animated films may reflect the difference between analytic and holistic thinking style.