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Health disparities among Hispanics are an important, yet less explored, research topic. Although existing literature has explored factors affecting Hispanics’ health behaviors, the study of their cultural values and the effects on their health behaviors remains less investigated. This study employed a questionnaire survey to examine the relationships among Hispanic cultural values, health beliefs, attitudes, and practices. Empirical results from two linear regression analyses found that Respeto and Familismo factor, as well as Personalismo factor, significantly predicted Hispanics’ health behaviors, while Machismo factor was not a significant predictor. Both theoretical and practical implications were discussed.