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Negative emotionality (NE) and positive emotionality (PE) have repeatedly shown to act as vulnerability factors for youth depression. Less research examined the mechanisms through which these temperament traits may differently confer vulnerability to depression. Based on recent cognitive-affective models proposing emotion regulation (ER) as a key underlying mechanism, current study aimed to clarify the general, momentary, and day-to-day relations among temperament, ER strategies, and depressive symptoms in Dutch-speaking youth (35% boys; M_age = 13.27 years, SD = 1.98) using a cross-sectional (n = 495) and a 7-day daily diary design (n = 469). Self-reported temperament, trait rumination, trait positive refocusing, and depressive symptoms were measured at baseline. Rumination, positive refocusing, and depressive symptoms were further assessed daily. The cross-sectional analyses provide preliminary evidence for the hypothesis that NE and PE each provide unique pathways for understanding vulnerability to depression whereas no such evidence was found for understanding momentary levels of depressive symptoms. Additional exploratory analyses in the daily diary study showed PE to be significantly related to trajectories of symptoms while NE was significantly related to trajectories of rumination. Results contribute to a more nuanced understanding of the associations between temperament, ER, and depressive symptoms in youth.