BackHome
Refresh: OffView Personal Schedule
Individual Submission Summary
Share...

Direct link:

4T1 murine breast cancer cell cytoxins in Rumex crispus (yellow dock)

Sat, March 7, 11:30am to 1:00pm, Wellness Center, Room 101

Abstract

The plant species Rumex crispus (yellow dock), an herbal remedy plant used in Turkey and the Far East has been shown to possess anti-microtubulin, anti-inflammatory, antimalarial activity. It has been used in the treatment constipation, diarrhea and eczema, and has been shown to have cytotoxicity and/or induction of apoptosis in T47D, MDA-MB-231 and MDA-MB-436 breast cancer cell and a variety of other cancer cell lines. Studies in our laboratory have shown that plant species (e.g., Ginger, Turmeric, Ashwaganda) with anti-inflammatory activity have cellular components cytotoxic for 4T1 murine breast cancer cells. To our knowledge, the cytotoxicity of yellow dock against 4T1 murine breast cancer cells has not been investigated. Yellow dock root powder was subjected to dichloromethane reflux (1 hr), and the resulting extract was resolved by Sephadex LH20 chromatography (75% ethanol). Fractions were pooled based upon UV-visible spectroscopic analysis (280 nm), and assayed for 4T1 cell cytotoxicity via MTS assay. Pooled cytotoxic fractions were further characterized via HPLC (C18, 0-75% methanol gradient over 30 min), and six chromatographic peaks were identified as cytotoxic to 4T1 cells (MTS assay). None of the six peaks identified co-migrated with emodin (270.24 g/mol) or chrysophanol (254.24 g/mol) standards, known bioactive components of yellow dock. Mass spectroscopic analysis (MALDI TOF) of the six captured HPLC peaks showed compounds of varying molar masses. Based upon MALDI analysis, none of the six peaks contained species that corresponded to emodin, chyrsophanol, nepodin (216.13) or bioactive anthroquinones previously identified. Further studies are underway to characterize these cytotoxic components.

Authors