Tumor growth monitoring of orthotopic colon cancer model

• In Event: Poster Presentation and Judging for: Biomedical; Cell & Molecular Biology; Chemistry & Biochemistry; Marine Science; Systematics & Evolutionary Biology.
  In Poster Session: Cell & Molecular Biology Poster session

Abstract

To explore the potential impact on colon cancer growth of low-dose chemotherapy regimens we generated orthotopic models of the human HT29 cancer cell line in immunodeficient mice. HT29 cells were transfected to express GFP (Green Flourescent Protein), and human chorionic gonadotropin (hCG), and subsequently injected intracaecally into R2G2KO SCID mice. Tumors were monitored via luminescence reading and assessment of hCG levels in mouse urine. Luminescence and hCG readings allow for a non-invasive form of monitoring tumor growth. The models were then treated with metronomic therapy of gemcitabine (GEM). The development of HT29 orthotopic models that are readily monitorable could improve the preclinical evaluation of new therapeutic strategies.

Authors

• jose lopez, University of Texas at El Paso

• Giulio Francia, University of Texas at El Paso