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The purpose of the study was to develop a scale with which to examine students’ self-regulation (SR) in three types of online interaction. Using accepted scale development steps, the authors constructed a self-report survey named the online self-regulation questionnaire (OSRQ). A total of 799 online students participated in the study. Data from a randomly selected half participants were used for exploratory factor analysis (EFA), and data from the other half were used for confirmatory factor analysis (CFA). The EFA yielded three factors as hypothesized: SR in interaction between (a) student and content, (b) student and student, and (c) student and teacher. The CFA demonstrated that the same factor structures of the OSRQ were observed with different participants.