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Acculturative stress may hamper students’ ability to succeed in the United States. Previous literature about the factors affecting Asian international students’ acculturative stress indicates mixed findings. This study analyzed the impact of a range of factors noted in the literature , and compared their stress levels before and during the pandemic of COVID-19 We collected three waves of data via an online survey from ten universities/colleges across different regions of the U.S. We selected two hundred and four responses for analyses. The results indicated education level, English proficiency, gender, and SES related to Asian international students’ acculturative stress. Moreover, Asian international students are undergoing more acculturative stress of being discriminated against and marginalized during the Covid-19 pandemic than before.