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Teachers turn to many sources for support with social media-based communities increasingly being an important factor in teachers’ professional learning. This study examines teacher collaboration in a Twitter community for Biology teachers (N=2,040 tweets, N=93 teachers). Using epistemic network analyses, we examined the collaborative structures in these communities to identify how participation can relate to high-quality features of teacher professional development. Results indicate that some teachers use Twitter with a content focus and coherent to their individual contexts and knowledge. Notably, differences in collaboration and participation patterns by teachers’ overall activity level hint at the existence of an online community of practice. Overall, this study illustrate how Twitter may serve as a relevant avenue for teacher learning.