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The purpose of this quantitative, predictive correlational study was to investigate the relationship between leader self-efficacy, emotional intelligence, years of experience, and transformational leadership practices of physician assistant (PA) program directors. Participants for this study included a sample of 86 PA program directors across the United States. Multiple linear regression was used to examine the predictive relationship between the variables. The findings revealed that there was a statistically significant predictive relationship between transformational leadership practices and the linear combination of leader self-efficacy, emotional intelligence, and years of experience. This study’s findings can be used to develop more targeted professional development to enhance PA program directors’ leadership effectiveness.