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Sexual assault case processing has been a longstanding issue of importance. Much of the research in this area has focused on factors that influence reporting, victim cooperation during investigations, and criminal justice system response to victims, highlighting the role of law enforcement agencies. The current study extends this line of research by examining the role prosecutors play in sexual assault case processing. Specifically, we assess the influence of DNA evidence on prosecutorial decision making in these cases. Thirty-eight prosecutors were surveyed regarding the processing of biological evidence in sexual assault cases; results from the dimensional analysis produce several themes related to the perspective, context, process, and conditions under which DNA evidence plays a vital role in sexual assault case processing.